Figure 7.

DR protects intestinal biological barrier from 5-FU.

Fecal samples were randomly collected from in young and old mice after two weeks of AL or DR diet before 5-FU treatment and gut microbiota were analyzed by 16S rRNA gene sequencing (n = 6 mice per group randomly picked from 2 independent experiments) and qPCR analysis (n = 5 mice per group randomly picked from 2 independent experiments).

(A,B) Principal-coordinate analysis (PCoA) based on Bray-Curtis distance showed a significant shift of the overall structure of the gut microbiota by DR in both young and old mice.

(C,D) Relative abundance of the intestinal microbiota of indicated groups on the order level showed by 16S rRNA gene sequencing.

(E,F) LDA scores in the fecal microbiomes of indicated groups. LDA score > 4 and top fifteen bacteria were shown.

(G-J) Relative abundance of the Lactobacillales and Betaproteobacteriales showed by 16S rRNA gene sequencing.

(K-N) qPCR analysis of the amounts of Lactobacillus and Proteus of indicated groups.

(G-N) Unpaired two-tailed, Student’s t test. Results were displayed as mean±SD. *p < .05; **p < .01;***p < .001; ns, not significant.