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. 2024 Apr 26;12(5):873. doi: 10.3390/microorganisms12050873

Figure 2.

Figure 2

Tight junction protein abundance and localisation. (a) Immunolocalisation of tight junction proteins occludin (red) and zona occludens-1 (ZO-1; green) in 17-day-old Caco-2 monolayers co-cultured with the indicated probiotic strains (multiplicity of infection of 1000) for 8 h. Scalebar, 25 µm. (b) Effect of probiotics on co-localisation of occluding and ZO-1 in Caco-2 cell monolayers. Graph shows the mean (+/− SEM) correlation (n = 6 confocal image stacks per treatment; 15–26 images per stack). (c) Effect of probiotics on abundance of occludin. Graph shows the mean (+/− SEM) grey value of occludin (n = 4 confocal image stacks per treatment). (d) Effect of probiotics on abundance of ZO-1. Graph shows the mean (+/− SEM) grey value of ZO-1 (n = 4 confocal image stacks per treatment). * p < 0.05, ** p < 0.01 compared to ‘no treatment’.