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. 2024 Apr 29;16(5):705. doi: 10.3390/v16050705

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection and distribution of CsPaV and CsPV in diseased Chinese tongue sole. (A) Distribution and copy numbers of CsPaV and CsPV in the liver, spleen, kidney, intestine, gills, and muscle. Data are the means and standard errors of the means for three replicates. (B) PCR detection of CsPaV and CsPV in the experimental group and control group. Lane M: DL2000 Marker; Lane 1 and 2: CsPaV and CsPV in homogenate for injection; Lane 3 and 4: CsPaV and CsPV in the kidney sample of control group; Lane 5 and 6: CsPaV and CsPV in kidney sample of experimental group; Lane 7 and 8: negative control of CsPaV and CsPV; Lane 9 and 10: positive control of CsPaV and CsPV. (C) Cumulative mortality of Chinese tongue soles infected with CsPaV and CsPV.