FIG. 1.

Disruption of mag1 in S. pombe. (A) The mag1::ura4 disruption construct was made as follows. A blunt-ended DNA fragment containing the S. pombe ura4 gene (hatched box) was ligated into a unique EcoRV site in the mag1 cDNA (shaded box) as diagrammed. The BcgI/BclI fragment was isolated, purified, and used to transform ura4D-18 leu1-32 S. pombe. (B) Genomic DNA from 5FOA-resistant S. pombe clones was isolated, digested with HindIII, and analyzed by Southern using radiolabeled mag1 cDNA as a probe. The positions and molecular sizes of marker DNA are indicated.