FIG. 2.

MMS sensitivity of 3MeA DNA glycosylase-deficient cells. The colony-forming ability of cells from various organisms following MMS treatment was determined. For E. coli, S. cerevisiae, and murine embryonic stem cells, doses and times are as indicated and data shown are adapted from references 8, 12, and 32. For S. pombe, 0.2% MMS was added to logarithmically growing wild-type (WT; TE236) or mag1 (AM006) S. pombe. Aliquots were collected at the indicated times and scored for colony-forming ability as described in Materials and Methods. The data shown for S. pombe are the mean from five independent experiments.