FIG. 3.

MMS sensitivity of S. pombe deficient in DNA repair. (A) MMS (0.2%) was added to logarithmically growing S. pombe. Aliquots were collected at the indicated times and scored for colony-forming ability as described in the Materials and Methods. The strains analyzed were wild-type (TE236), mag1 (AM006), rad2 (TE793), rad13 (TE791), and rhp51 (TE792) S. pombe. The data shown are the mean and standard error from three experiments for rad2, rad13, and rhp51 and five experiments for wild type and mag1. (B) MMS gradient plate analysis was performed on the indicated strains as described in Materials and Methods. The concentration of MMS indicated reflects the concentration of the bottom layer of agar and corresponds to the highest concentration of the gradient.