FIG. 6.
MMS sensitivity of S. pombe overexpressing S. cerevisiae AP endonuclease. MMS gradient plate analysis was performed on wild-type, rhp51, and mag1 rhp51 S. pombe strains expressing either the pREP vector plasmid or the pREP-APNsc plasmid expressing the S. cerevisiae AP endonuclease gene APN1 as described in Materials and Methods. The MMS concentration (0.005%) reflects the concentration of the bottom layer of agar and corresponds to the highest concentration of the gradient. The strains and plasmids are as indicated. Similar results were obtained from three independent experiments using two independent transformants for each strain.