Figure 7.
Affinity of 80S-BaDV-2 IGR IRES complexes. Filter binding assays. [32P]-BaDV-2 IGR IRES, [32P]-(CrPV) IGR IRES or mutant (∆PKI/II/III) CrPV IGR IRES were incubated with increasing amounts of purified salt-washed 80S. The fractions bound were quantified by filter binding assay followed by phosphorimager analysis. CrPV WT has a KD value of 1.4 nM, CrPV mutant (MT, mutations that disrupt all three pseudoknot base-pairings) has a KD value of 321.0 nM and WT BaDV-2 has a KD value of 940.3 nM. Shown are the averages from at least three independent experiments ± standard deviation.