Figure 9.

Effects of translation inhibitors on BaDV-2 IRES translation. (A) Schematic illustration of bicistronic reporter RNAs with CrPV or BaDV-2 IGR IRESs. In vitro transcribed reporter RNAs were incubated (B) in RRL with hippuristanol or (C) in Sf21 extracts with NSC119889 at the indicated concentrations. RLuc and FLuc activities were measured and normalized to DMSO-treated lysates with wild-type CrPV or BaDV-2 IGR IRES bicistronic RNAs. A one-way ANOVA statistical test was used to determine the p values. * p < 0.05, ** p < 0.01. “n.s.” denotes the difference is not significant between the control groups and the experimental groups (p > 0.05). Shown are the averages from at least three independent experiments ± standard deviation.