. 2024 May 13;16(5):226. doi: 10.3390/toxins16050226

Table A4.

Polyunsaturated fatty acid profile and calculated indices of the renal phospholipids.

Fatty Acid	Exp. Day	Control				Low Mix				High Mix				Sig.	O. Power
C18:2n6	D_0	11.3	±	2.19	a	11.3	±	2.19		11.3	±	2.19	a
	D_1	12.1	±	0.46	ab	13.0	±	1.78		12.0	±	1.08	ab	0.292	0.244
	D_2	11.6	±	0.84	ab	12.7	±	1.98		14.0	±	3.17	b	0.196	0.32
	D_3	13.1	±	1.62	b	12.7	±	2.86		11.9	±	1.08	ab	0.565	0.13
Sig.		0.083				0.957				0.151
O. power		0.489				0.055				0.371
C18:3n6	D_0	0.07	±	0.03	a	0.07	±	0.03	a	0.07	±	0.03	a
	D_1	0.08	±	0.01	a	0.08	±	0.02	a	0.08	±	0.01	a	0.785	0.082
	D_2	0.08	±	0.02	aA	0.07	±	0.01	aA	0.10	±	0.01	bB	0.006	0.881
	D_3	0.11	±	0.02	bB	0.11	±	0.02	bAB	0.09	±	0.01	abA	0.09	0.474
Sig.		0.005				0.006				0.002
O. power		0.893				0.877				0.948
C18:3n3	D_0	0.03	±	0.01		0.03	±	0.01		0.03	±	0.01	ab
	D_1	0.03	±	0.00	B	0.02	±	0.00	A	0.02	±	0.01	aAB	0.082	0.491
	D_2	0.02	±	0.00	A	0.02	±	0.00	A	0.03	±	0.01	bB	0.007	0.867
	D_3	0.02	±	0.00		0.02	±	0.01		0.02	±	0.00	a	0.348	0.212
Sig.		0.076				0.41				0.062
O. power		0.505				0.183				0.546
C20:2n6	D_0	0.75	±	0.22		0.75	±	0.22		0.75	±	0.22
	D_1	0.79	±	0.04		0.75	±	0.10		0.73	±	0.11		0.564	0.131
	D_2	0.73	±	0.05	A	0.81	±	0.06	AB	0.89	±	0.15	B	0.033	0.659
	D_3	0.86	±	0.17		0.82	±	0.09		0.71	±	0.12		0.167	0.351
Sig.		0.147				0.323				0.049
O. power		0.376				0.226				0.589
C20:3n9	D_0	0.40	±	0.21		0.40	±	0.21		0.40	±	0.21
	D_1	0.37	±	0.09		0.31	±	0.23		0.43	±	0.14		0.453	0.166
	D_2	0.39	±	0.16		0.32	±	0.10		0.36	±	0.28		0.814	0.077
	D_3	0.30	±	0.16		0.36	±	0.21		0.39	±	0.14		0.699	0.098
Sig.		0.572				0.863				0.819
O. power		0.129				0.069				0.076
C20:3n6	D_0	4.40	±	1.46		4.40	±	1.46		4.40	±	1.46
	D_1	4.44	±	0.83		3.94	±	0.62		4.12	±	0.45		0.421	0.179
	D_2	4.60	±	0.52		4.12	±	0.29		4.68	±	0.72		0.191	0.325
	D_3	4.43	±	0.58		4.41	±	0.38		4.62	±	0.35		0.672	0.104
Sig.		0.889				0.232				0.168
O. power		0.065				0.288				0.35
C20:4n6	D_0	21.3	±	2.29	b	21.3	±	2.29	b	21.3	±	2.29	b
	D_1	21.2	±	0.69	b	19.7	±	2.30	b	19.9	±	0.64	b	0.175	0.341
	D_2	19.7	±	1.21	bB	21.5	±	1.44	bB	10.3	±	1.17	aA	<0.001	1
	D_3	12.0	±	2.47	a	11.3	±	1.63	a	12.3	±	0.99	a	0.656	0.108
Sig.		<0.001				<0.001				<0.001
O. power		1				1				1
C20:3n3	D_0	0.03	±	0.02		0.03	±	0.02		0.03	±	0.02
	D_1	0.03	±	0.00		0.03	±	0.01		0.03	±	0.01		0.971	0.054
	D_2	0.03	±	0.01		0.03	±	0.01		0.04	±	0.02		0.536	0.139
	D_3	0.04	±	0.02		0.03	±	0.01		0.02	±	0.01		0.289	0.246
Sig.		0.361				0.859				0.341
O. power		0.206				0.07				0.216
C20:4n3	D_0	0.02	±	0.01		0.02	±	0.01		0.02	±	0.01	a
	D_1	0.03	±	0.01		0.02	±	0.01		0.03	±	0.01	b	0.26	0.266
	D_2	0.03	±	0.01		0.03	±	0.00		0.03	±	0.01	ab	0.511	0.147
	D_3	0.03	±	0.01		0.03	±	0.01		0.03	±	0.01	b	0.775	0.083
Sig.		0.501				0.224				0.921
O. power		0.15				0.294				0.06
C20:5n3	D_0	0.27	±	0.05	b	0.27	±	0.05	c	0.27	±	0.05	c
	D_1	0.23	±	0.04	ab	0.19	±	0.05	b	0.22	±	0.01	c	0.204	0.312
	D_2	0.22	±	0.03	aB	0.25	±	0.06	bAB	0.13	±	0.04	aA	0.001	0.972
	D_3	0.16	±	0.04	a	0.15	±	0.03	a	0.18	±	0.03	b	0.25	0.274
Sig.		0.014				0.006				0.001
O. power		0.786				0.88				0.972
C22:4n6	D_0	1.37	±	0.14	b	1.37	±	0.14	b	1.37	±	0.14	b
	D_1	1.31	±	0.09	b	1.23	±	0.19	ab	1.35	±	0.09	b	0.32	0.228
	D_2	1.33	±	0.09	bB	1.31	±	0.22	abB	0.96	±	0.23	aA	0.007	0.866
	D_3	1.01	±	0.10	a	1.10	±	0.23	a	1.08	±	0.14	a	0.611	0.119
Sig.		<0.001				0.294				0.003
O. power		1				0.243				0.939
C22:5n6	D_0	1.31	±	0.36	b	1.31	±	0.36	b	1.31	±	0.36	b
	D_1	1.14	±	0.28	b	1.23	±	0.34	ab	1.26	±	0.18	b	0.736	0.091
	D_2	1.27	±	0.36	bB	1.14	±	0.33	bAB	0.70	±	0.21	aA	0.014	0.789
	D_3	0.77	±	0.18	a	0.89	±	0.22	a	0.89	±	0.17	a	0.504	0.149
Sig.		0.02				0.162				<0.001
O. power		0.735				0.357				0.992
C22:5n3	D_0	0.22	±	0.03		0.22	±	0.03		0.22	±	0.03
	D_1	0.26	±	0.02		0.24	±	0.05		0.23	±	0.03		0.541	0.138
	D_2	0.28	±	0.07		0.24	±	0.04		0.22	±	0.03		0.138	0.388
	D_3	0.25	±	0.07		0.25	±	0.04		0.22	±	0.02		0.413	0.182
Sig.		0.758				0.855				0.464
O. power		0.087				0.07				0.162
C22:6n3	D_0	1.25	±	0.25	b	1.25	±	0.25	b	1.25	±	0.25	b
	D_1	1.14	±	0.20	b	1.22	±	0.20	b	1.17	±	0.15	b	0.747	0.089
	D_2	1.34	±	0.15	bB	1.25	±	0.15	bB	0.68	±	0.14	aA	<0.001	1
	D_3	0.80	±	0.12	a	0.76	±	0.08	a	0.82	±	0.15	a	0.663	0.106
Sig.		<0.001				<0.001				<0.001
O. power		0.998				1				0.999
total n3	D_0	1.82	±	0.26	b	1.82	±	0.26	b	1.82	±	0.26	b
	D_1	1.71	±	0.22	b	1.72	±	0.23	b	1.71	±	0.17	b	0.987	0.052
	D_2	1.92	±	0.23	bB	1.81	±	0.14	bB	1.12	±	0.18	aA	<0.001	1
	D_3	1.31	±	0.20	a	1.23	±	0.13	a	1.29	±	0.18	a	0.735	0.091
Sig.		<0.001				<0.001				<0.001
O. power		0.987				0.999				0.999
total n6	D_0	40.5	±	2.63	b	40.5	±	2.63	b	40.5	±	2.63	b
	D_1	41.1	±	0.48	b	40.0	±	2.53	b	39.4	±	1.40	b	0.271	0.258
	D_2	39.3	±	1.71	bB	41.7	±	1.50	bB	31.7	±	2.71	aA	<0.001	1
	D_3	32.3	±	2.76	a	31.4	±	2.03	a	31.6	±	1.02	a	0.739	0.09
Sig.		<0.001				<0.001				<0.001
O. power		1				1				1

a,b,c: lower case letters indicate differences within the group between consecutive experimental days (p < 0.05); A,B: capital letters indicate differences between groups on each individual experimental days; Sig.: significance, indicating the p-value. O. power: observed power, representing the probability of detecting a true effect when it exists. Low-mix” group: T-2/HT-2 toxin: 0.25 mg/kg; DON/3AcDON/15-AScDON: 5 mg/kg; FB₁: 20 mg/kg feed; high-mix group: T-2/HT-2 toxin: 0.5 mg/kg, DON/3-AcDON/15-AcDON: 10 mg, FB₁:40 mg/kg feed.