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. 2024 Apr 30;16(5):717. doi: 10.3390/v16050717

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Stimulation profile of CD4⁺ T lymphocytes cultured with the MDA-MB 231 tumor cell line. The experimental groups were composed of the empty pcDNA vector and lymphocytes cultured with MDA-MB 231 transfected with the E5, E6, and E7 genes. (A)—Differential count of CD4⁺ T lymphocytes. Differential (B) count of the CD25⁺ subset of CD4⁺ T lymphocytes and its suppressor expression profile by the presence of the intracellular molecule FOXP3⁺. (C–E) The presence of IFN-γ, IL-17, and IL-10 intracellular cytokines in CD4⁺ lymphocytes, respectively. ** p < 0.01, *** p < 0.001, **** p < 0.0001. Error bars: standard error between samples.