Figure 2.

Coordinative repression and induction of ccn1 and ccn2 transcripts in response to inflammatory TNF-α (A) and pro-inflammatory TGF-β (B) in chondrocytic HCS-2/8 cells. Results of mRNA quantification by real-time RT-PCR analysis are displayed. Plotted values represent relative mRNA copy numbers versus those at time 0. Each copy number was computed by standardizing the raw data against those of β-actin. Open circles, ccn1; solid circles, ccn2. Mean values and standard deviations (SDs) of at least 2 experiments are shown.