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. 2024 May 20;12(5):559. doi: 10.3390/vaccines12050559

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Spike mRNA in situ hybridization (ISH) detected at site of injection and draining lymph nodes 1 day after IM Ad26.COV2.S dosing in rabbits. Anti-SARS-CoV2 S1 staining by immunohistochemistry (IHC) and ISH of spike mRNA at (A) the administration site and in (B) lymph nodes from rabbits (n = 4 per group) at Day 1 after dosing with Ad26.COV2.S (positive) or Ad26.Empty (negative) or ISH on Day 11 after dosing with Ad26.COV2.S (negative). The bacterial gene dihydrodipicolinate reductase (dapB) (negative control) and housekeeping gene cyclosporine-binding protein peptidylpropyl isomerase B (PPIB) (positive control) are shown in the same tissues. In brown (IHC) are the positive areas for the target protein and in purple/red are the positive areas for the target mRNA (ISH). The black bar represents the magnification of the image.