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. 2005 Apr 27;4:21. doi: 10.1186/1475-2875-4-21

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Copyright © 2005 Lavstsen et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PfEMP1 expression in trophozoite-stage cultures of unselected 3D7 (3D7_UM), 3D7 selected for expression of VSA_SM-type IE surface antigens (3D7_SM) and of NF54 established from six volunteers on different days after infection. A: Western blot using antibodies (αATS) targeting the acidic terminal segment (ATS), which is conserved between most PfEMP1 types. Black arrows indicate changes in protein expression between isolates of the same volunteer. B: Western blot identifying the αATS-detected 400 kDa band in the day 10 culture of volunteer 1 as PFD1235w/VAR4 using an αDBL5δ antibody. VAR4 is expressed on the surface of the 3D7_SMline selected for high immune serum recognition [17].