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. 2024 May 6;16(5):736. doi: 10.3390/v16050736

Figure 6.

Figure 6

G2/M phase arrest is required for SeMNPV replication in P8-Se301-C1 cells. P8-Se301-C1 cells synchronized to G2/M (A) and G1 (B) phases were infected with SeMNPV (MOI = 1) for 1 h and then cultured in a medium containing 7 μg/mL nocodazole to arrest cells in the G2/M phase. qRT-PCR was performed to measure the BV production in the culture supernatant at the indicated time points. The infected cells cultured in a medium without nocoodazole were used as the control. (C) Light microscopy of SeMNPV infected P8-Se301-C1 cells at specific cell cycle phases. P8-Se301-C1 cells were synchronized at the G1 phase, S phase, and G2/M phase and then infected with SeMNPV (MOI = 1). In addition, SeMNPV-infected G1 and G2/M cells were treated with nocodazole to further arrest the infected cells in the G2/M cells. SeMNPV-infected, unsynchronized Se301 and P8-Se301-C1 cells were used as controls. Bar = 50 μm. Data are presented as the mean ± standard deviation in biological triplicate experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.