Table 6.
List of primers used in reverse transcription quantitative-PCR gene expression analysis in weaned piglets.
| Genes | GenBank number | Sequence2 |
|---|---|---|
| GPX | NM_214201.1 | F: 5′GCCCAACTTCATGCTCTTC3′ |
| R: 5′CAGGATCTCCCCATTCTTGGC3′ | ||
| SOD | NM_001190422.1 | F: 5′ATCAAGAGAGGCACGTTGGA3′ |
| R: 5′TCTGCCCAAGTCATCTGGTT3′ | ||
| CAT | NM_214301.2 | F: 5′GCTTTAGTGCTCCCGAACAG3′ |
| R: 5′AGATGACCCGCAATGTTCTC3′ | ||
| OCL | NM_001163647.1 | F: 5′TCCTGGGTGTGATGGTGTTC3′ |
| R: 5′CGTAGAGTCCAGTCACCGCA3′ | ||
| ZO-1 | XM_003353439.2 | F: 5´AAGCCCTAAGTTCAATCACAATCT3′ |
| R: 5´ATCAAACTCAGGAGGCGGC3′ | ||
| IFN- γ | NM_213948 | F: 5′TGGTAGCTCTGGGAAACTGAATG3′ |
| R: 5′GGCTTTGCGCTGGATCTG3′ | ||
| TNF-α | NM_214022.1 | F: 5′CATCGCCGTCTCCTACCA3′ |
| R: 5′CCCAGATTCAGCAAAGTCCA3′ | ||
| IL1- β | NM_214055.1 | F: 5′TCTGCCCTGTACCCCAACTG3′ |
| R: 5′CCCAGGAAGACGGGCTTT3′ | ||
| IL-10 | NM_214041.1 | F: 5′GAAGGACCAGATGGGCGACTT3′ |
| R: 5′CACCTCCTCCACGGCCCTTG3′ | ||
| GLUT2 | FJ209732.1 | F: 5′TTGCCTTGGATGAGTTATGTGA3′ |
| R: 5′GCGTGGTCCTTGACTGAAAA3′ | ||
| SGLT1 | MW_280290.1 | F: 5′GGTTGGAGCTTCTCTGTTTG3′ |
| R: 5′CCAGAACAACCACCCAAATC3′ | ||
| SMCT2 | XM_003122908.1 | F: 5′AGGTCTACCGCTTTGGAGCAT3′ |
| R:5′GAGCTCTGATGTGAAGATGATGACA3′ | ||
| MCT1 | AM_286425.1 | F: 5′GGTGGAGGTCCTATCAGCAG3′ |
| R: 5′AAGCAGCCGCCAATAATCAT3′ | ||
| PepT1 | AY_180903.1 | F: 5′CATGGATGCTGTGGTGTATC3′ |
| R: 5′CATGGAAGCCAGGAACATC3′ | ||
| EAAC1 | NM_001164649.1 | F: 5′CAGTGGATGCCATGTTAGAC3′ |
| R: 5′CGTCTCTGGCTCACTAGAA3′ | ||
| y+LAT1 | EU_047705.1 | F: 5′TGCGTGGAGGACATCTT3′ |
| R: 5′CTCCTTCCAGCGCAAATAG3′ | ||
| β-actin | U07786.1 | F: 5′CTCTTCCATCGTGTCCTTCTAC3′ |
| R: 5′CCTCAGACTTGTCGATCTTCTG3′ |
GPX glutathione peroxidase, SOD superoxide dismutase, CAT catalase, OCL occluding, ZO-1 zonula occludens-1, IFN-γ interferon gamma, TNF-α tumor necrosis factor alpha, IL1-β interleukin 1 beta, IL-10 interleukin 10, GLUT2 glucose transporter type 2, SGLT1 Na + /glucose co-transporter 1, SMCT2 sodium-coupled monocarboxylate transporter, MCT1 monocarboxylate transporter 1, PepT1 peptide transporter 1, EAAC1 excitatory amino acid carrier 1, y + LAT1 Na + -independent cationic and Na + -dependent neutral amino acid transporter 1. 2F and R indicate Forward and Reverse primers, respectively.