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. 2023 May 20;16(3):936–948. doi: 10.1007/s12602-023-10085-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characteristics of S. boulardii EVs. NTA particle size distribution analysis (A) and TEM image of EVs (B); representative histograms of the average size distribution from three measurements of a single sample (black line). The numbers show the maxima of particular peeks, and widened areas indicate the standard deviation (SD) between measurements. Average protein and phospholipid content in S. boulardii EVs (C). The size parameters of the EVs measured by NTA; factors D10, D50, and D90 mean that 10%, 50%, and 90% of the EV population had a diameter of less than or equal to the given value (D)