Table 2.

Δ. Comparison of cytokines secretion by wild type, Mincle- or Myd88 KO primary macrophages in response to infection with BCG or BCGΔBCG1419c.

TNF-α (pg/mL)
WT			Mincle KO			Myd88 KO
BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented
14,685.25 ± 2022.29	12,694.92 ± 2463.62	11,330.98 ± 2321.5	6101.66 ± 1108.66	4737.03 ± 1213.91	4206.09 ± 1057.24	917.235 ± 216.03	745.483 ± 138.99	937.91 ± 69.9
			p < 0.0001 (WT vs Mincle KO)	p = 0.0001 (WT vs Mincle KO)	p = 0.0095 (WT vs Mincle KO)	p = 0.0095 (WT vs Myd88 KO)	p = 0.0023 (WT vs Myd88 KO)	p = 0.0095 (WT vs Myd88 KO)
Values shown are the means with standard deviations						p = 0.0022 (Mincle KO vs Myd88KO)	p = 0.0004 (Mincle KO vs Myd88KO)	p = 0.00046 (Mincle KO vs Myd88KO)
Mean fold-change vs WT			2.4	2.7	2.7	6.6	6.3	4.5

IL-6 (pg/mL)
WT			Mincle KO			Myd88 KO
BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented
6370 ± 1122.04	5128 ± 1099.71	5050.15 ± 1158.33	3114.88 ± 878.28	2214.77 ± 828.76	2120.979 ± 687.01	Not determined (ND, below detection limits)	ND	ND
			p < 0.0001 (WT vs Mincle KO)	p = 0.0001 (WT vs Mincle KO)	p = 0.0095 (WT vs Mincle KO)	ND	ND	ND
Values shown are the means with standard deviations
Mean fold-change vs WT			2	2.3	2.4

G-CSF (pg/mL)
WT			Mincle KO			Myd88 KO
BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented	BCG	BCGΔBCG1419c	Complemented
5639.35 ± 206.47	5339.43 ± 349.16	5412.86 ± 184.42	1570.77 ± 525.94	1055.72 ± 458.25	1048.38 ± 434.76	59.35 ± 4.53	59.38 ± 6.26	58.39 ± 4.53
			p < 0.0001 (WT vs Mincle KO)	p < 0.0001 (WT vs Mincle KO)	p < 0.0001 (WT vs Mincle KO)	p < 0.0001 (WT vs Myd88 KO)	p < 0.0001 (WT vs Myd88 KO)	p < 0.0001 (WT vs Myd88 KO)
Values shown are the means with standard deviations						p = 0.0009 (Mincle KO vs Myd88 KO)	p = 0.0031 (Mincle KO vs Myd88 KO)	p = 0.0026 (Mincle KO vs Myd88 KO)
Mean fold-change vs WT			3.6	5.1	5.2	95	89.9	92.7