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. 2024 May 24;14:11909. doi: 10.1038/s41598-024-62871-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Timeline of dynamic impacts of radiation on CD8 T cell recirculation to the TdLN. (a) (i) Kaede mice were implanted with MC38 tumors and at d14 the tumor was left untreated or was irradiated with 12 Gy CT-guided radiation using a SARRP. In addition, at d14, 18, or 21 the tumor was selectively photoconverted with UV light. The TdLN were harvested 1 day following photoconversion, resulting in analysis of recirculation rates to the TdLN 1, 5, or 8 days following radiation. (ii) The proportion of CD8 T cells that are photoconverted in the TdLN at each time point. Key. NS not significant; *p < 0.05.