Figure 4.

Copper does not influence volume and sphericity of PrP condensates.A, image quantifications of the condensates formed by MoPrP and MoN1 shown in Figure 3, D and E. Droplet surfaces were reconstructed using Imaris 10.1.0 surface module; droplet volume and sphericity were plotted against different CuCl₂ concentrations. Statistical analysis: Kolmogorov-Smirnov test followed by ANOVA with Dunnett’s Multiple Comparison Test (n = 7 per condition from four independent experiments). B, the table summarizes the names, abbreviations, and amino acid sequences of the constructs used in this study. Mouse sequences are highlighted in blue and Xenopus laevis sequences in green. The octarepeat is marked in gray and the analyzed tryptophanes and histidines are shown in bold letters. Mutations are depicted in red.