Table 1.

Cryo-EM data collection parameters for the different samples

Samples	Number of micrographs	Total dose (e−/Å2)	Physical pixel size (Å)	Spherical aberration (mm)	Frame	Nominal defocus range (μm)
HCN3-apo	3390	50	0.842	2.7	32	−1 to −2
HCN3-cAMP	4720	40	0.668	2.7	32	−1 to −2

Abbreviation: HCN, Hyperpolarization-Activated Cyclic Nucleotide-gated.