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. 2024 May 15;121(21):e2321410121. doi: 10.1073/pnas.2321410121

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Copyright © 2024 the Author(s). Published by PNAS.

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Fig. 2. — Neuronal PAS domain 1 (Npas1)-expressing neurons of the basal forebrain (BF) are GABAergic neurons distinct from parvalbumin (PV) and cholinergic (choline acetyltransferase, ChAT) neurons. (A) The vast majority of immunohistochemically stained NPAS1⁺ nuclei (red) colocalize with green fluorescent protein (GFP) in GAD67-GFP knock-in mice. NPAS1⁺ nuclei (green) do not colocalize with tdTomato (red) in (B) vesicular glutamate transporter 1 (vGlut1)-cre-tdTomato or (C) vesicular glutamate transporter 2 (vGlut2)-cre-tdTomato mice. (D and E) Npas1-tdTomato+ neurons (red) do not colocalize with neurons stained against ChAT (green, D) or PV (green, E).