Fig. 4.

High-confidence PPIs in synaptic signaling. (A) PrePPI and ZEPPI predict interactions among Sharpin (the Shank-interacting protein-like 1, colored in green), the small GTPase K-Ras (orange), and Shank1 (the SH3 and multiple ankyrin repeat domains protein 1, colored in blue). Solid lines between protein domains denote the domains involved in the PPIs which are depicted as backbone ribbons. In B–D, the darker colored ribbons represent the chains from the PDB PPI template and are defined below the query protein names: (B) Sharpin-K-Ras; (C) K-Ras-Shank1; and (D) Sharpin-Shank1. In all but two cases (KRAS-6ba6:B and KRAS-5o2t:A), the pairwise sequence identities between the queries and the respective template chains are less than 25%.