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. 2024 May 3;32(2):200812. doi: 10.1016/j.omton.2024.200812

Figure 5.

Figure 5

hCHST15 siRNA diminishes MDSCs in the TDLN

(A) Concentrations of hCHST15 siRNA within mouse tissues were detected by HLPC. Typical histograms of hCHST15 siRNA in the local tissue (pancreas) and its draining LN (paraaortic LN) 1 h after single local injection of hCHST15 siRNA are shown. Negative control shows HPLC result in the pancreas 1 h after single local injection of saline. (B) Quantitative analysis for CHST15-immunostained stained tissues of TDLN from human BxPC-3 model (left) or mouse KPC model (right). Percentage positive areas for CHST15 are shown. Mean ± SD (n = 15 for BxPC-3 model, n = 5 for KPC model). ∗∗p < 0.01, ∗∗∗p < 0.001 by Mann Whitney test. (C) Immunostaining for Ly6C/G (brown) in the TDLN of KPC model (left panels). Original magnification ×100. Yellow arrows indicate positive signals. Quantitative analysis for stained tissues (right). Percentage positive areas for Ly6C/G are shown. Mean ± SD (n = 5). ∗∗p < 0.01 by Mann-Whitney test. LN, lymph node.