TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Primer sequencea | Hybridization region |
|---|---|---|
| AJ286 | cgggcatATGCCCAAAGTAATTTATG | 5′ end of fdx3 |
| NdeI | ||
| AJ287 | gcgcggatccGTTCAATCAAGATTGTTG | 3′ end of fdx3 |
| BamHI | ||
| AJ316 | ccccggatccatgatgcggccGCCAGATTCTAGACCGGGATCAG | 3′ end of redA2 |
| BamHINotI | ||
| AJ317 | cgcggaaTTCTGGAAGAGAAGGAAAG | 5′ end of redA2 |
| EcoRI | ||
| AJ320 | ccgggcggccGCTCAATCTGATGCACCA | 5′ end of fdx3 |
| NotI | ||
| AJ321 | cgccggaTCCCATTAGAGATTACATCT | 3′ end of fdx3 |
| BamHI |
a
Relevant recognition sequences for the restriction enzymes are underlined, and lowercase characters indicate the nonhomologous sequences to the corresponding DNA region from Sphingomonas sp. strain RW1.