TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype | Source |
|---|---|---|
| E. coli | ||
| EK370 | MC4100/pLAN701 (ompR of E. coli cloned into pACYC177) | 31 |
| EK371 | MC4100/pLAN702 (ompRD55E of E. coli cloned into pACYC177) | 31 |
| EK372 | MC4100/pLAN801 (ompR of E. coli cloned into pUC19) | 31 |
| Serovar Typhimurium | ||
| SF241(CH511) | LT2 oppBCD250 leu-1151::Tn10 ompR::Tn5 | 18 |
| SF261(TT10287) | LT2 hisD::MudJ his-9941::Mud1a | 25 |
| SF463(TT10423) | LT2 proAB47/F′ pro+ lac+ zzf-1831::Tn10D16D17Tcr | K. Sanderson |
| SF464(TT10427) | LT2/pNK972 (Tn10 transposase; Apr) | K. Sanderson |
| SF465(TT10604) | LT2 proAB47/F′ pro+ lac+ zzf-1836::Tn10Δ16Δ17Cmr | K. Sanderson |
| SF500 | 14028S rpoS::pRR10-250V (Apr) | F. Fang |
| SF530(χ3761) | Wild-type UK1 | 7 |
| SF586(JR501) | hsdSA29 hsdSB121 hsdL6 metA22 metE551 trpC2 ilv-452 rpsL120 xyl-404 galE719 H1-6 h2 -e, n, x nml (Fels2)−fla-66 | 5 |
| SF680(TE6153) | puPA1303::Kmr -katE-lac(op) | 4 |
| SF681(JS91) | 14028S ack408::Tn10dTc | 54 |
| SF682(JS105) | 14028S pta209::Tn10dTc | 54 |
| SF790(CH1118) | LT2 oppBCD250 leu3051 envZ::MudJ | K. Sanderson |
| SF797 | LT2 supD-zeb-609::Tn10 ompF1004::MudJ | B. Finlay |
| SF798 | SL1344 ompC396::Tn10 ompF1006::MudA tppB83MudJ | B. Finlay |
| SF819 | SF586/pRDQ55 (ompRD55Q of E. coli cloned into pBR322) | 39 |
| YK3092 | UK1 ompR132::MudJ | SF261 × SF530 |
| YK3230 | UK1 ompR::Tn5 | SF241 × SF530 |
| JF2651 | LT2 ompC9::MudA | |
| JF2757 | UK1 ompR43::MudJ | SF261 × SF530 |
| JF3063 | UK1 ompR43::MudJ rpoS::Ap | SF500 × JF2757 |
| JF3266 | UK1 putPA1303::Kmr -katE-lac(op) | SF680 × SF530 |
| JF3269 | UK1 ack408::Tn10dTc | SF681 × SF530 |
| JF3271 | UK1 pta209::Tn10dTc | SF682 × SF530 |
| JF3298 | UK1 ack408::Tn10dTc putPA1303::Kmr -katE-lac(op) | SF680 × JF3269 |
| JF4240 | UK1 envZ::MudJ (Lac−) | SF790 × SF530 |
| JF4289 | UK1 ompC9::MudA | JF2651 × SF530 |
| JF4290 | UK1 ompC9::MudA envZ::MudJ (Lac−) | JF2651 × JF4240 |
| JF4336 | UK1 ompR43::MudJ ack408::Tn10dTc | JF2757 × JF3269 |
| JF4337 | UK1 ompR43::MudJ pta209::Tn10dTc | JF2757 × JF3271 |
| JF4339 | UK1 ack408::Tn10dTc putPA1303::Kmr -katE-lac(op)/pNK972 (Tn10 transposase; Apr) | SF464 × JF3298 |
| JF4344 | UK1 ompF1004::MudJ | SF797 × SF530 |
| JF4352 | UK1 ompF1004::MudJ ompC396::Tn10 | SF798 × JF4344 |
| JF4369 | UK1 ack408::Tn10dTet pta::Tn10dCm putPA1303::Kmr -katE-lac(op) | (SF465 × JF4339) × JF3266 |
| JF4370 | UK1 ompR43::MudJ ack408::Tn10dTet pta::Tn10dCm | JF4369 × JF2757 |
| JF4371 | UK1 ompR43::MudJ/pLAN701 [OmpREC] | EK370 × JF2757 |
| JF4372 | UK1 ompR43::MudJ/pLAN702 [OmpRECD55E] | EK371 × JF2757 |
| JF4373 | UK1 ompR43::MudJ/pLAN801 [OmpREC] | EK372 × JF2757 |
| JF4414 | UK1 ack-408::Tn10dTet pta::Tn10dCm | JF4369 × SF530 |
| JF4419 | UK1 ompR43::MudJ ack-408::Tn10dTet pta::Tn10dCm/pLAN701 | EK370 × JF4370 |
| JF4420 | UK1 ompR43::MudJ ack408::Tn10dTet pta::Tn10dCm/pLAN702 | EK371 × JF4370 |
| JF4450 | UK1 ompR43::MudJ/pRDQ55 | SF819 × JF2757 |
| JF4452 | UK1 ompR43::MudJ ack408::Tn10dTet pta::Tn10dCm/pRDQ55 | SF819 × JF4370 |
| JF4479 | UK1 ompR43::MudJ/pAC2005S | pAC2005S × JF2757 |
| JF4481 | UK1 ompC9::MudA ack-109::Tn10dTc pta::Tn10dCm | JF2651 × JF4414 |
| JF4482 | UK1ompC9::MudA ack-109::Tn10dTc pta::Tn10dCm envZ::MudJ | JF4240 × JF4481 |
| Plasmids | ||
| pAC2005s | EnvZEC in pACYC184 | 52 |
| pLAN701 | OmpREC in pACYC177 | 31 |
| pLAN702 | OmpRECD55E in pACYC177 | 31 |
| pLAN801 | OmpREC in pUC19 | 31 |
| pNK972 | pBR333 Apr containing Tn10 transposase | 43 |
| pRDQ55 | OmpRECD55Q in pBR322 | 39 |
a
Mud1 and MudA confer Apr; MudJ confers Kmr.