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. 2024 May 25;20:228. doi: 10.1186/s12917-024-04079-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Proportion of seropositive cattle for each grid cell using MNT and cELISA kits with different thresholds to determine the number of positive sera. EL1: ID Screen® cELISA, EL2: Immunozym cELISA, A: cELISA-positive and -doubtful serum according to the threshold recommended by the manufacturer confirmed to be MNT-positive (scenario A); B: cELISA-positive serum according to the adjusted threshold weighting sensitivity and specificity and confirmed MNT-positive (scenario B), C: cELISA-positive serum according to the adjusted threshold weighting sensitivity and specificity (scenario C)