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. 2024 May 25;20:228. doi: 10.1186/s12917-024-04079-8

Table 1.

Relative sensitivity (Se), relative specificity (Sp), kappa coefficient (ƙ) and area under the curve (AUC) of the two cELISA (ID Screen® cELISA, Immunozym cELISA) kits compared with the reference MNT test on 428 cattle samples

ID Screen® cELISA Immunozym cELISA
Cutoff 50 86 63 62
positive negative positive negative positive negative positive negative
MNT positive 31 170 130 71 151 50 156 45
MNT negative 2 225 56 171 22 205 22 205
Se (%) 15.4 64.5 75.1 78.1
Sp (%) 99.1 75.3 90.3 90.3
ƙ 0.11 0.40 0.68 0.68
AUC (%) 78 89

Cutoffs 50 and 63: doubtful thresholds recommended by the manufacturers; cutoffs 86 and 62: optimal positive thresholds weighting sensitivity and specificity