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. 2024 May 22;65(5):36. doi: 10.1167/iovs.65.5.36

Figure 2.

Figure 2.

Identification of CD38 KO mice and assessment of retinal morphology and visual function. (A) CD38 gene knockout schematic. (B) PCR identification of CD38 KO mice. (C) CD38 immunofluorescence staining of retinal paraffin sections identified successful CD38 knockdown. (D) Immunofluorescent staining of Rbpms in retinal flat preparations for assessing the number of RGCs in WT and CD38−/− mice. (E) H&E staining of paraffin sections of retinas from WT and CD38 KO mice was observed using an inverted fluorescence microscope at a magnification of × 20. (F) Quantitative analysis of retinal thickness in WT and CD38 KO mice. (G) Quantitative statistical analysis of the average, central, middle, and peripheral RGCs in the retina of WT and CD38−/− mice. (H, I) Dark-adapted 3.0 ERG assays in WT and CD38 KO mice. (J, K) Dark-adapted 3.0 oscillatory potential ERG analysis in WT and CD38 KO mice. (L, M) Bright adapted 3.0 ERG analysis in WT and CD38 KO Mice. Peripheral, about 150 to 200 µm from the retinal margin; middle, about 700 to 800 µm from the margin; and central, about 1500 to 1600 µm from the margin; WT, wild type; KO, knockout; Rbpms, markers for RGCs; data presented as: mean ± SEM, ns, not significant.