Figure 8.

To evaluate the effect of CD38 deletion on NAD+ content, acetylated protein content, and inflammatory and apoptotic protein expression in the retina in the optic nerve crush model. (A) In the crush day 7 model, the NAD+ kit was used to detect the changes of NAD + content in the retina of WT and KO mice. (B) In the crush day 7 model, the expression of Sirt1 protein in the retina of WT and KO mice was detected by WB. (C) In the crush day 7 model, the expression of Ac-p65 protein in the retina of WT and KO mice was detected by WB. (D) In the crush day 7 model, the expression of Ac-p53 protein in WT and KO retinas was detected by WB. (E) In the crush day 7 model, the expression of TNF-α protein in the retina of WT and KO mice was detected by WB. (F) In the crush day 7 model, WB was used to detect the changes of IL-1β protein expression in the retina of WT and KO mice. (G) In the crush day 3 model, WB was used to detect changes in Caspase3 protein expression in the retina of WT and KO mice. WT, wild type; KO, knockout; data presented as: mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.