TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| T. ferrooxidans | ||
| ATCC 33020 | Isolated from a uranium mine in Japan | ATCCa |
| ATCC 19859 | Isolated from acid copper leaching water in Canada | ATCC |
| Tf-49 | Isolated from a coal mine in Yunqin (China) | 24 |
| BRGM1 | Isolated twice on DOP medium from the BRGM strain | 8 |
| E. coli | ||
| HB101 | Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 Δ(mcr-mrr) rpsL20 xyl-5 mtl-1 recA13 | |
| MC1061 | araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL hsdR2(rK− mK+) mcrA mcrB1 | |
| MOS Blue | endA1 hsdR17(rK− mK+) supE44 thi1 gyrA46 recA1 lac/F′ (lacIqZΔM15 proAB Tn10) | |
| S17-1 | recA pro hsdR (RP4-2 Tc::Mu Km::Tn7) | 31 |
| CGSC7330 | pro-81::Tn10 rph-1 | E. coli Genetic Stock Center |
| Plasmids | ||
| pNG23 | recA-recX-alaS region cloned in the HindIII site of pUC19; identical to pNG22 (14) with the insert in the opposite orientation | |
| pHP45Ω-Km | pHP45 plasmid carrying the Ω-Km cassette | 13 |
| pUC18mob | pUC18 plasmid in which an Sau3A fragment carrying the mob region of the RP4 plasmid has been inserted in the BamHI site | 31 |
| RP4 | Apr Tcr Kmr IncP Tra+ | 10 |
| pKT240 | Apr Kmr (Tn903) IncQ Mob+ | 4 |
| pJRD215 | Smr Kmr (Tn5) IncQ Mob+ | 11 |
| pJB3Km1 | Apr Kmr (Tn903) IncP Mob+ | 7 |
| pUFR034 | Kmr (Tn903) IncW Mob+ | 12 |
a
ATCC, American Type Culture Collection.