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. 2024 Apr 8;9(7):e172678. doi: 10.1172/jci.insight.172678

Figure 1. Lower FLRT2 expression levels in senescent endothelial cells and aged rat and human aortic tissues.

Figure 1

(A–C) Young (passage 4) and old (passage 15) human umbilical vein endothelial cells (HUVECs) (A), endothelial colony-forming cells (ECFCs) (B), and human microvascular endothelial cells (HMVECs) (C) were subjected to senescence-associated β-galactosidase (SA-β-Gal) and immunoblot analyses; SA-β-Gal–positive cells were quantified. Scale bar: 10 μm. The values represent mean ± SD (n = 3; ***P < 0.001). (D) Aortas of 6- and 24-month-old rats were subjected to immunoblot assays. (E) Changes in the expression levels of CD31 and FLRT2 in human arterial tissues of each age group (n = 20) were detected using immunofluorescence. Scale bar: 50 μm. The values represent mean ± SD (n = 20; **P < 0.01). Two-tailed t test.