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. 2024 Apr 8;9(7):e172678. doi: 10.1172/jci.insight.172678

Figure 6. Overexpression of FLRT2 rescues the vascular aging effect of silencing FLRT2 on endothelial cell senescence.

Figure 6

(A–C) HUVECs exogenously expressing empty vector or Flag-FLRT2 were transfected with Con Si or FLRT2 Si. Immunoblot assay was performed with the indicated antibodies (A), and relative cell number (B) and SA-β-Gal activity (C) were assessed at 3 days after siRNA transfection. The values represent the mean ± SD (n = 3; *P < 0.05; **P < 0.01). One-way ANOVA. (D) Representative immunoblot images obtained from aortic lysates of mice injected intravenously with Con Si, FLRT2 Si, empty vector, or Flag-FLRT2 plasmid (n = 5). (E) Image of SA-β-Gal–stained aortas of mice. Scale bar: 5 mm. (F) Immunostaining for p-AKT (S473), p53, and p21 in aortas of mice. Scale bar: 100 μm.