Figure 6. Proinflammatory phenotype of migrated human Tregs on protein levels.

HD-derived migrated and nonmigrated Tregs were collected and either directly phenotyped or phenotyped after coculture with Teff. (A–D, F, and H) Using flow cytometry, molecules identified by pathway analysis were validated. MFI of CCR6 (A), MFI of CCR4 (B), MFI of IL-6R (C), MFI of TNF-α (D), percentage of IFN-γ⁺ cells (F), or MFI of AREG (H) of live cells are shown. Horizontal bars represent group mean. n = 5–6; One-tailed Wilcoxon test. Gating in Supplemental Figure 9. (E and G) Concentration of IFN-γ (E) and IL-10 (G) were measured in supernatants of Teff/Treg cocultures (1:0 or 1:1). n = 5; Friedman test with Dunn’s multiple-comparison test. *P < 0.05