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. 2024 Feb 22;9(7):e167457. doi: 10.1172/jci.insight.167457

Figure 8. Proinflammatory CD49d+ Tregs accumulate in the CSF of people with MS.

Figure 8

(A and B) Frozen PBMCs of HD and people with different MS types were thawed and studied using flow cytometry (gating in Supplemental Figure 11). (A) FlowSOM analysis on CD4 T cells identified 2 subpopulations of Tregs. (B) Heatmap related to the FlowSOM analysis. Color scale as percentiles. n = 26 (HD), n = 24 (uRRMS), n = 25 (flRRMS), n = 23 (uSPMS). (C) Fresh, paired blood, and CSF samples were collected from persons with uRRMS at diagnosis and studied using flow cytometry. Percentage expression of different migratory molecules within single, live, CD4+CD25hiCD127lo Tregs illustrated that CD49d+ Tregs are enriched in the CSF. Gating in Supplemental Figure 12. Horizontal bars represent mean of the group. n = 5; 2-way ANOVA with Bonferroni’s multiple-comparison test. ***P < 0.001.