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. 2024 Mar 5;9(7):e169830. doi: 10.1172/jci.insight.169830

Figure 8. CD93 deficiency impairs extracellular matrix deposition in the primary tumor site and at the metastatic lungs.

Figure 8

Representative immunofluorescent staining of fibronectin (green) (A) and collagen IV (green) (B), in the primary HCmel12 tumor vasculature of wild-type and CD93–/– mice. Vasculature is detected by CD31 staining (red). Nuclei are visualized by Hoechst (blue). (C and D) Quantification of fibronectin+ and collagen IV+ area in primary HCmel12 tumor vasculature (n = 9/group, minimum of 6 fields of view/sample). **P < 0.01; ***P < 0.001 by 2-tailed t test. (E and F) Representative immunofluorescence images of fibronectin (green) and collagen IV (green) in the metastatic lungs of wild-type and CD93–/– mice. Metastatic lesions of mCherry-HCmel12 tumor cells (TC, gray) are defined by a dotted line and the vasculature is detected by CD31 staining (red). Nuclei are visualized by Hoechst (blue). (G and H) Quantification of fibronectin+ and collagen IV+ area around the metastatic lesions in wild-type and CD93–/– lungs (n = 9/group, minimum of 3 fields of view/sample). Scale bars: 20 μm and 10 μm (high-magnification insets in A and B). AU, arbitrary units. **P < 0.01; ***P < 0.001 by 2-tailed t test. Values represent mean ± SEM.