TABLE 2.

MICs for E. coli DH5α bearing the pBAD24 vector with various inserts^a

Compound	MIC (μg/ml) with insert
	None	YkkC	YkkD	YkkC-YkkD
Cationic dyes
Ethidium bromide	50	50	50	2,000
Proflavine	20	20	20	500
Tetraphenylarsonium chloride	200	200	200	1,000
Crystal violet	2	2	2	50
Pyronin Y	5	5	5	500
Methyl viologen	50	50	50	1,000
Cetylpyridinium chloride	50	50	50	500
Neutral antimicrobial
Chloramphenicol	2	2	2	10
Other antimicrobials
Streptomycin	2	2	2	100
Tetracycline	0.5	0.5	0.5	2
Anionic antimicrobial
Phosphonomycin	0.1	0.1	0.1	10

^aThe proteins produced by expression in pBAD24 (all SMR genes are from Bacillus subtilis) are as follows: none (no insert); YkkC (ykkC, gi 2632029); YkkD (ykkD, gi 2632030); and YkkC/YkkD (both the ykkC and ykkD genes are expressed under the control of the single P_BAD promoter).