Figure 6.

IFN-γ induced production of IL-32 in PFMCs. (A) PFMCs isolated from fresh TPE were treated with IFN-γ (20 ng/mL), IL-1Ra (20 ng/mL), and TNF-α (20 ng/mL) for 24 hours; mRNA expression of the IL-32 was measured by quantitative PCR (mean fold change ± SEM, n = 6). (B) PFMCs isolated from fresh TPE were stimulated with IFN-γ (20 ng/mL), IL-1Ra (20 ng/mL), and TNF-α (20 ng/mL) for 24 hours. Total IL-32 production was measured by ELISA 24 hours later in cells lysed with Triton X-100 (mean fold change ± SEM, n = 6). *p < 0.01; ***p < 0.001. PFMCs, pleural fluid mononuclear cells; TPE, tuberculous pleural effusion.