Figure 1.

Development of a cellular atlas of the murine small bowel using high-parameter flow cytometry. (A) A schematic summarizing our experiment design is shown. (B) The total cell counts that are obtained using our two cell isolation strategies are shown. (C) Cell viability reflected as the percentage of dead cells is shown. (D) The distributions of total cell count and cell viability are plotted by sex for each isolation strategy. (E) A representative gating strategy used to enumerate live cells and four major cell lineages is provided. (F) An MDS plot illustrating effect of genotype (marker colors) and sex (marker shapes) on the abundance of lineage⁺ cells is shown. (G) The absolute abundance of lineage⁺ cells are shown across genotypes. (H) An atlas of 59 cellular phenotypes that can be discriminated using our SI-customized high-parameter flow cytometry approach is provided. (B, C, G) Dunnett’s Test (“all vs. WT”), ns=p>0.05, *=p<0.05, **=p<0.01, ***=p<0.001, ****=p<0.0001. (D, F) Unpaired Student’s t-test, ns=p>0.05, ****=p<0.0001. (A, H) Mouse figures created with Biorender (Biorender.com).