Figure 2.

PAD-induced immune dysregulation is primarily associated with defects in T cell homeostasis in the small bowel. (A) PAD phenotypes and anticipated degree of immune dysregulation (size of circles) expected in mouse strains utilized in this study. Figure created with Biorender (Biorender.com). (B) The degree of immune dysregulation (i.e. divergence in immune phenotype from the WT condition) among mouse strains is shown. Dunnett’s Test (“all vs. WT”), *=p<0.05, ****=p<0.0001. (C) A representative tSNE map of cellular subsets identified using our general immunophenotyping panel is shown. (D) Density tSNE plots highlighting differences in cellular distributions among genotypes are shown (each plot is generated from concatenated events from six mice per genotype). T and B cell deficient RAG1^-/- and CD4-deficient MHCII^-/- mice are provided as gating controls. (E) An MDS plot illustrating immunophenotypic variation among mouse strains (left), and the relative contribution of innate versus adaptive immune responses in driving immune dysregulation (right) are shown. Mann-Whitney U test, *=p<0.05. (F) The results of response screening analysis of immune phenotypes that significantly correlate (denoted by red bars) with the degree of immune dysregulation are shown. Significance reflects results of FDR-corrected p-values from multiple linear regression analysis of all 19 general immune cell subsets.