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. 2024 May 13;15:1278197. doi: 10.3389/fimmu.2024.1278197

Figure 3.

Figure 3

PAD-induced immune dysregulation is associated with enhanced type I immunity and diminished iNKT cell responsiveness. (A) A representative tSNE map of identifiable conventional T cell subsets is shown. (B) Density tSNE plots highlighting differences in absolute T cell subset abundances are shown (each plot is generated from concatenated events from six mice per genotype). CD4-deficient MHCII-/- mice are provided only as gating controls. (C) Statistical comparisons of the absolute abundance of TH1, TH17, and TH2 cell between WT and PAD mice are shown. Kruskal-Wallis test, *=p<0.05, ns=p>0.05. (D) The results of response screening analysis of T cell phenotypes that significantly correlate (denoted by red bars (left panel)) with the degree of immune dysregulation are shown. Significance reflects results of FDR-corrected p-values from multiple linear regression analysis of all 10 T cell subsets (top 5 most significant correlations shown (right panel)). (E) A representative tSNE map of identifiable iNKT cell subsets is shown. (F) Density tSNE plots highlighting differences in absolute iNKT cell subset abundances are shown (each plot is generated from concatenated events from six mice). CD4-deficient MHCII-/- mice are provided only as gating controls. (G) The results of response screening analysis of iNKT cell phenotypes that significantly correlate (denoted by red bars (left panel)) with the degree of immune dysregulation are shown. Significance reflects results of FDR-corrected p-values from multiple linear regression analysis of all 10 iNKT cell subsets (top 5 most significant correlations shown (right panel)). (H) An MDS plot illustrating immunophenotypic variation among mouse strains based on T and iNKT cell phenotypes (left), and the relative contribution of effector T cell and effector iNKT cell responses in driving immune dysregulation (right) are shown. Mann-Whitney U test, ***=p<0.001. Significant correlations (FDR-corrected p-values <0.05) between T cell and iNKT cell subsets with immune dysregulation are shown.