FIG. 1.

Analysis of the proteins secreted by different Salmonella strains (sipB mutants). The secreted proteins deposited by each strain into proteinaceous filaments were isolated as described earlier (17). The protein samples from serovar Dublin B1 (lane 1), Salmonella enterica serovar Gallinarum S9.B1 (lane 2), Salmonella enterica serovar Enteritidis S13.B1 (lane 3), serovar Typhimurium F98.B1 (lane 4), serovar Typhimurium ST4/74.B1 (lane 5), serovar Typhimurium ST5306.B1 (lane 6), and Salmonella enterica serovar Choleraesuis A57.B1 (lane 7) were separated by SDS–12% PAGE and were stained with Coomassie brilliant blue (A) or were transferred onto nitrocellulose membrane and probed with an anti-SopE monoclonal antibody (B). Molecular mass is shown on the left.