FIG. 3.

Western blot analysis of the secreted proteins of the wild-type serovar Typhimurium F98 (lane 2), serovar Typhimurium SE2.1 sopE2 mutant (lane 3), and serovar Typhimurium SE2.1/pSopE2st transcomplemented sopE2 mutant (lane 4) strains. Culture supernatant proteins from different Salmonella strains were prepared by precipitation with 10% trichloroacetic acid as previously described (17), were separated by SDS–PAGE, were transferred to nitrocellulose membrane, and were probed with anti-SopE2 monoclonal antibody.