Table 5. Selectivity of Selected BTK Inhibitors over Kinases with a Reactive Cysteine at the 481 Position.

			compound
	amino acid @484 position	tolebrutinib (2) Kd [nM]	evobrutinib (4) Kd [nM]		10Kd [nM]	25Kd [nM]	27Kd [nM]
BTK Kd [nM]	Asn	1.4	4.6	0.6		0.63	0.31
Kinase with Cys481		Fold Selectivity for BTK (off-target kinase Kd/BTK Kd)
BMX	Asn	3	4	43		43	14
TEC	Asn	1	<1	9		9	7
TXK	Asn	5	37	27		27	6
JAK3	Asp	847	1217	157		157	390
BLK	Asp	3	133	460		460	839
ITK	Asp	671	587	1200		1206	274
EGFR	Asp	9	2000	>10 000		>10 000	9355
ERBB2	Asp	2	1152	>10 000		>10 000	7419
ERBB4	Glu	4	137	700		714	7741