Fig. 3.

Sex-dependent (a-g) lung mRNA gene expression levels of cytokines in response to acute WS exposure. Lung mRNA relative gene expression levels demonstrated a response influenced by sex when exposed to WS. Data were analyzed using a two-way ANOVA, with (h) indicating the P value for each variable (sex, treatment, and interactions between sex and treatment) on lung mRNA genes, where P < 0.05 signified statistical significance. Asterisks denote statistically significant mean differences between groups, as identified by Tukey’s post-hoc test (*P < 0.05). Data are presented as mean ± SEM. Each treatment group consisted of n = 8 mice. Mice across all groups were euthanized 24 h post-exposures