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. 2024 May 27;15:4514. doi: 10.1038/s41467-024-48901-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a 3D live imaging of a GALNT6-Halo-3xPA KI cell transfected with mNeonGreen-golgin-84 by SCLIM (Supplementary Movie 9). Left: A low magnification micrograph of the Golgi near the nucleus (Nu). A region in the small rectangle is magnified in the right panels. Right: Top panels show GALNT6, golgin-84 and merged micrographs at 0 sec. Middle and bottom panels show the movement of GALNT6 in the Golgi unit surrounded by golgin-84 from 3 to 18 sec. A large part of the GALNT6 signal is localised near or on the rim of the unit. Frame rate is 3 seconds/frame (3 seconds/volume). Bar, 5 µm (left), 1 µm (right). b 3D live imaging of a XYLT2-Halo-3xPA KI cell transfected with mNeonGreen-golgin-84 (Supplementary Movie 10). Left: a low magnification micrograph of the Golgi near the nucleus (Nu). A region in the small rectangle is magnified in the right panels. Right: Top panels show XYLT2, golgin-84 and merged micrographs at 0 sec. Middle and bottom panels show the movement of XYLT2 in the Golgi unit surrounded by golgin-84 from 3 to 18 sec. Frame rate is 3 seconds/frame. Bar, 5 µm. c 3D live imaging of an XYLT2-Halo-3xPA + GALNT6-2xSNAP-9xMyc double KI cell (Supplementary Movie 13). Left: a low magnification micrograph of the Golgi near the nucleus (Nu). A region in the small rectangle is magnified in the right panels. Right: Top panels show XYLT2, GALNT6, and merged micrographs at 0 sec. Middle and bottom panels show the movements of XYLT2 (magenta) and GALNT6 (green) in the Golgi unit from 3 to 18 sec. They are localised almost mutually exclusively. Frame rate is 3 seconds/frame. Bars, 5 µm (left), 1 µm (right).