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. 2024 May 27;15:4505. doi: 10.1038/s41467-024-48758-4

Fig. 1. Isolation and characterization of human H7N9 mAbs in vitro.

Fig. 1

a FACS depicting the staining and selection of H7-specific B cells from donor H7N9_HK2013 PBMCs. SSC-A, side scatter area; FSC-A, forward scatter area. b ELISA binding curves of the indicated mAbs to soluble recombinant H7N9 HA, with and without Endo H treatment, to H7N9 HA1 from 2013, 2016, and 2017, to H7N7 HA, and to 6 non-H7 HA or HA1. c Western blot of a cleaved H7 HA (molecular mass of 43 kDa for HA1 and 30 kDa for HA2) with mAb H7.HK2 or H7.HK4. d Neutralization curves of H7.HK mAbs against H7N9 2013 and 2017 pseudo viruses in MDCK cells. Data shown are mean ± SEM. Source data are provided in the Source Data file. Similar results were independently reproduced at least once.