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. 2024 May 14;15:1404047. doi: 10.3389/fendo.2024.1404047

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Copyright © 2024 Chiariello, Conte, Rossetti, Trofarello, Salvioli and Conte

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blotting analysis of GDF15 in the skeletal muscle (SM-GDF15). (A) Representative immunoblotting image of GDF15 and GAPDH in the skeletal muscle. (B) Relative protein expression of GDF15 in the SM from 23 healthy subjects (HS) and 16 patients with lower limb mobility impairment (LLMI). (C) Relative protein expression of GDF15 in the SM from 10 HS <40 years (<40) and 13 HS >70 years (>70). (D) Relative protein expression of GDF15 in the SM from 7 LLMI <40 and 9 LLMI >70. The bars represent mean ± SD. The quantification was performed using Fiji software and normalized to GAPDH expression. Student’s t test was applied.