Figure 1. sFlt-1 reduces neurite growth in RA-differentiated SH-SY5Y cells.

RA-differentiated SH-SY5Y cells were treated with 0.1, 1, 10, or 100 ng/ml sFlt-1 or PBS as vehicle control, daily for 72 h. (A) Graph and (B) representative photomicrographs of neurite length. (C) Graph of extracellular LDH activity as a measure of cytotoxicity. Data are mean + SEM from five independent experiments (n=5) expressed as percentage of the control. One-way ANOVA and post-hoc Dunnett’s test (**P<0.01 vs. control).