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. 2024 May 17;44(5):BSR20240562. doi: 10.1042/BSR20240562

Figure 5. ReNcell VM express machinery for endogenous VEGFA autocrine and paracrine signalling, which is unchanged by sFlt-1 treatment.

Figure 5

ReNcell® VM were differentiated into neurons and glia over 15 days while exposed to 100 ng/ml sFlt-1, or PBS as vehicle control. (A) Timeline of experiment: on day 1 (undifferentiated) and day 15 (differentiated), RNA was extracted, and relative mRNA expression measured by RT-qPCR. (B–D) Graphs of (B) VEGFA, (C) VEGFR-2, and (D) sFlt-1/VEGFR-1 mRNA expression at DIV1. (E–G) Graphs of (E) VEGFA, (F) VEGFR-2, and (G) sFlt-1/VEGFR-1 mRNA expression at DIV15. Data are mean + SEM from three independent experiments (n=3) expressed as fold-change of the control group mean. Student’s paired t-test.